Kurzweil, Lisa; Stark, Timo D.; Hille, Karina; Hoheneder, Felix; Mrtva, Jana; Hausladen, Johann; Lenk, Miriam; Motawia, Mohammed Saddik; Strittmatter, Nicole; Vlot, A. Corina; Pillen, Klaus; Sorensen, Mette; Moller, Birger L.; Hueckelhoven, Ralph; Dawid, Corinna UPLC-ESI-TOF-MS Profiling of Metabolome Alterations in Barley (Hordeum vulgare L.) Leaves Induced by Bipolaris sorokiniana Journal Article In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 73, no. 39, pp. 24662-24687, 2025, ISSN: 0021-8561. @article{WOS:001574260900001,
title = {UPLC-ESI-TOF-MS Profiling of Metabolome Alterations in Barley (Hordeum vulgare L.) Leaves Induced by Bipolaris sorokiniana},
author = {Lisa Kurzweil and Timo D. Stark and Karina Hille and Felix Hoheneder and Jana Mrtva and Johann Hausladen and Miriam Lenk and Mohammed Saddik Motawia and Nicole Strittmatter and A. Corina Vlot and Klaus Pillen and Mette Sorensen and Birger L. Moller and Ralph Hueckelhoven and Corinna Dawid},
doi = {10.1021/acs.jafc.5c05419},
issn = {0021-8561},
year = {2025},
date = {2025-10-01},
urldate = {2025-10-01},
journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
volume = {73},
number = {39},
pages = {24662-24687},
abstract = {Spot blotch of barley (Hordeum vulgare L.), caused by Bipolaris
sorokiniana, is responsible for major losses in crop yield.
Breeding-resistant barley varieties have proven to be an effective
countermeasure for protecting agricultural production. Plants react to
pathogen attacks by up-regulating secondary metabolites. Marker
compounds for a B. sorokiniana infection are examined by untargeted
UPLC-TOF-MS metabolomics and lipidomics techniques. Through the analysis
of nine quantitatively resistant and susceptible barley genotypes,
derived from the nested association mapping population HEB-25, followed
by structure identification experiments and spore germination assays, 57
metabolites are identified. In addition to previously known metabolites,
the unknown compounds 5-carboxydidehydroblumenol C-9-O-ss-d-glucoside
(46) and grasshopper ketone 3-sulfate (47) were elucidated.
5-Carboxyblumenol C-9-O-ss-d-glucoside (45) was described for the first
time in barley leaves. Pheophytin derivatives, oxylipins,
linolenate-conjugated lipids, and flavone glycosides were described for
the first time in connection with infections by phytopathogenic fungi or
resistance in barley.},
keywords = {Lipids, Metabolomics, MS imaging, Plant stress},
pubstate = {published},
tppubtype = {article}
}
Spot blotch of barley (Hordeum vulgare L.), caused by Bipolaris
sorokiniana, is responsible for major losses in crop yield.
Breeding-resistant barley varieties have proven to be an effective
countermeasure for protecting agricultural production. Plants react to
pathogen attacks by up-regulating secondary metabolites. Marker
compounds for a B. sorokiniana infection are examined by untargeted
UPLC-TOF-MS metabolomics and lipidomics techniques. Through the analysis
of nine quantitatively resistant and susceptible barley genotypes,
derived from the nested association mapping population HEB-25, followed
by structure identification experiments and spore germination assays, 57
metabolites are identified. In addition to previously known metabolites,
the unknown compounds 5-carboxydidehydroblumenol C-9-O-ss-d-glucoside
(46) and grasshopper ketone 3-sulfate (47) were elucidated.
5-Carboxyblumenol C-9-O-ss-d-glucoside (45) was described for the first
time in barley leaves. Pheophytin derivatives, oxylipins,
linolenate-conjugated lipids, and flavone glycosides were described for
the first time in connection with infections by phytopathogenic fungi or
resistance in barley. |
